TABLE 2.
Primers used in this study
| Method and gene, plasmid, or strain | Primer | Sequence (5′ to 3′) |
|---|---|---|
| For RT-PCR | ||
| Amplified region 1 | 570-560_F | ATAGCCATGAAAGGGATGCG |
| 570-560_R | AGGAGAACGAGGATTTCCGC | |
| Amplified region 2 | 560-550_F | CGGCAGATATTTCTCCAGCC |
| 560-550_R | CCTTGATGCTACAGATCGGC | |
| Amplified region 3 | 550-540_F | GTGTGCGTCGTCTCGATG |
| 550-540_R | TGCTGGAGACGATGGTCG | |
| Amplified region 4 | 540-530_F | AGGATTGCGGTTTCCACCAT |
| 540-530_R | TACTGCGAGCACTGGTACAC | |
| Amplified region 5 | 530-520_F | GCGTTGATTTCCACCAGTCC |
| 530-520_R | ATGGTGGAAACCGCAATCCT | |
| Amplified region 6 | 520-510_F | CGTTTCCTCCACCAGCTTCT |
| 520-510_R | GGACTGGTGGAAATCAACGC | |
| Amplified region 7 | 510-500_F | CGTGAAACCCGCATCGA |
| 510-500_R | CATGTCGAGGTGCAGGTACT | |
| Amplified region 8 | 500-490_F | CTGTTCGGCGTGAAGAGATG |
| 500-490_R | GCCTATCTCCAGCACCAGTT | |
| Amplified region 9 | 490-480_F | CAGCACGAAATCGAACGGTC |
| 490-480_R | CATCTCTTCACGCCGAACAG | |
| For plasmid construction | ||
| pAK06550 | 06550top_F | GCAAGCTTCCTGTGCGCCTTCATCG |
| 06550bottom_R | GATATTTCTCCAGCCAGCCGAATTCGCTGGAACTCCAGCTATGGTCG | |
| pAK06550 and pAK06540 | 06550bottom-06540top_F | GCGAATTCGGCTGGCTGGAGAAATATC |
| 06550bottom-06540top_R | CGCTTTCCAGCTCATAATGGGATCCCGTGGAACTGGCAGTAATAGGG | |
| pAK06540 and pAK06530 | 06540bottom-06530top_F | CGGGATCCCATTATGAGCTGGAAAGCG |
| 06540bottom-06530top_R | CCCTCGATGGTGATGACCTCTAGACGCGTGTCGAGATCGTTCGG | |
| pAK06530 and pAK06520 | 06530bottom-06520top_F | CGTCTAGACGGTCATCACCATCGAGGG |
| 06530bottom-06520top_R | CACTTCGCCATGCTCCACGAATTCGCGCGTTGATTTCCACCAGTC | |
| pAK06520 and pAK06510 | 06520bottom-06510top_F | GCGAATTCGTGGAGCATGGCGAAGTG |
| 06520bottom-06510top_R | ATTGATGCGGCATTCGATGGGTACCGCATCGTCCAGATCCTCGACC | |
| pAK06510 and pAK06500 | 06510bottom-06500top_F | GCGGTACCCATCGAATGCCGCATCAAT |
| 06510bottom-06500top_R | GGCAACTGGTGCTGGAGATTCTAGACGTGGGGCTCAGCGTGAAGAC | |
| pAK06500 and pAK06490 | 06500bottom-06490top_F | CGTCTAGAATCTCCAGCACCAGTTGCC |
| 06500bottom-06490top_R | GTCTCGACCTTGGGATCAGGATCCCGCTTGTAGCCGCCCAGATTG | |
| pAK06490 | 06490bottom_F | CGGGATCCTGATCCCAAGGTCGAGAC |
| 06490bottom_R | GCGGTACCTGATGCGGGCGGAAATCTC | |
| pE21acv | acvA-acvF_F | AAGGAGATATACAGCGGCCGCGCGCGGAAAAGAGCATATGA |
| acvA-acvF_R | GCTCGAATTCGGATCTTATGCCAGCGGGAATGC | |
| pE16acvA | pE16acvA_F | TCGAAGGTCGTCATATGAGCGAACCGACCAAG |
| pE16acvA_R | GTTAGCAGCCGGATCCTCATGCGTCCTCCAGAAC | |
| pE16acvB | pE16acvB_F | TCGAAGGTCGTCATATGACGGCCGCCGTCTCC |
| pE16acvB_R | GTTAGCAGCCGGATCCTTATCTGCGTCCCCCGAA | |
| pE16acvF | pE16acvF_F | TCGAAGGTCGTCATATGACCGCCCCCTTCACC |
| pE16acvF_R | GTTAGCAGCCGGATCCTCAGCCCAGCAGGCCGAG | |
| pE16acvCDE | pE16acvCDE_F | TCGAAGGTCGTCATATGAGCCTCACGGCAAAG |
| pE16acvCDE_R | GTTAGCAGCCGGATCCTCAGCCATGGCTTTCCAG | |
| pQFacvCDE | pQFacvCDE_F | CTAGTAGAGGAAGCTATGAGCCTCACGGCAAAGGA |
| pQFacvCDE_R | TCACTTCACCGGATCTCAGCCATGGCTTTCCAGTT | |
| pQFacvCDEF | pQFacvCDE_F | CTAGTAGAGGAAGCTATGAGCCTCACGGCAAAGGA |
| pQFacvCDEF_R | TCACTTCACCGGATCTCAGCCCAGCAGGCCGAGCG | |
| pSEVA241_Plac | pSEVA241_Plac_F | TCACACAGGAGGCCGAGCGCCCAATACGCAAACC |
| pSEVA241_Plac_R | GCGCGGCCGCGGCCTCGTAATCATGGTCATAGCTG | |
| pQFvce | pQFvce_F | TGGTCTGTTTGTAACTAGTAGAGGAGCGATTGTAAAAAGTTAAGTAACACACTAAGGAGGTATTTTTATGGCCAAGACCTTCATCAC |
| pQFvce_R | TTTTTTTTTGCGGGTCACTTCACCGTCAGGCAGCGGAGCCGAACA | |
| pTS093vce | pTS093vce_F | TATCCTGCAGGAATTGCGATTGTAAAAAGTTAAGTAA |
| pTS093vce_R | CACCGTACGTCTCGATCAGGCAGCGGAGCCGAA | |
| For colony PCR | ||
| ΔacvR | dis06550_conf_F | ACGGCGATGACGATCAGCTC |
| dis06550_conf_R | CGTTGATGATGCGGTGATCG |