Table 1.
Bacterial strains, primers and plasmid used in this study.
| Bacterial strains | Relevant features | Reference |
|---|---|---|
| B. pseudomallei K96243 wild type | Human clinical isolate from Thailand; Ipms Cazs Chls Cips, Augmentins Minr Genr Tmp-Smxr Strr | 46 |
| B. pseudomallei 1026b wild type | Human clinical isolate from septicemic melioidosis from Thailand | 47 |
| B. pseudomallei K96243 Δhcp1 | Unmarked intragenic deletion in BPSS1498 | 48 |
| B. pseudomallei K96243 ΔbicA | Unmarked deletion of BPSS1533 | This study |
| B. pseudomallei K96243 ΔbicA::bicA | Unmarked deletion of BPSS1533, complemented by an unmarked insertion of BPSS1533 | This study |
| B. pseudomallei ΔtonB/Δhcp1 (PBK001) | Live-attenuated vaccine strain | 48 |
| E. coli NEB-5α | Competent DH5α-derived E. coli strain | New England Biolab |
|
E. coli S17-1 λpir |
Donor strain contain: TpR SmR recA, thi, pro, hsdR-M+ RP4: 2-Tc:Mu: Km Tn7 λpir |
|
| Primers |
Sequence |
Reference |
| pMo130-Forward | GACGAAAGGGCCTCGTGATAC | This study |
| pMo130-Reverse | TCGCGCGTTTCGGTGATG | This study |
| 1533 -Upstream-Forward | GTCATCACCGAAACGCGCGAGCCGTCATTCGGCATGCC | This study |
| 1533- Upstream-Reverse | CTCCTGTTGGGCCGTCACCTCGTTTGGTTC | This study |
| 1533-Downstream-Forward | AGGTGACGGCCCAACAGGAGCTGATCGTATGTCATCGGGAGTGCAGG | This study |
| 1533-Downstream-Reverse |
TATCACGAGGCCCTTTCGTCTCGGCCGCGTCGGCCGCC |
This study |
| Plasmid |
Relevant feature |
Reference |
| pMo130 | Suicide vector for allelic exchange in Burkholderia; ColE1 ori, RK2 oriT, xylE, sacB, KmR | 49 |