Figure 1.

A, Peripheral blood mononuclear cells from the patient (white bar, patient) and healthy volunteers (black bar, control, n = 7) were stimulated with LPS, P3C, Poly I:C and Mycobacterium abscessus (NTM), or RPMI. Culture supernatants were harvested, and concentrations of IL-6 and TNF-α were measured. Data were pooled from 3 experiments. *P < .05 based on Mann Whitney U test. B, Sanger sequence demonstrating the heterozygous cytosine C to thymine T switch at the c.1012 exon 11 of TRAF3 in the proband and location of R338W in the predicted structure by domains of TRAF3. Abbreviations: IL-6, interleukin 6; LPS, lipopolysaccharide; NTM, nontuberculous mycobacteria; P3C, Pam3Cys; TNF-α, tumor necrosis factor alpha.