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. 2022 Aug 18;14(1):2112882. doi: 10.1080/19490976.2022.2112882

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Silencing intestinal TLR4 aggravates intestinal barrier injury and bacterial translocation during acute pancreatitis.

(a) Photomicrograph of Occludin, Claudin1 and ZO-1 immunofluorescence in the ileum (100× magnification). The tight junction proteins/DAPI fluorescence ratio per field of view was measured. (b) The apoptosis in the small intestines was assayed by TUNEL. The number of TUNEL-positive cells (green) per field of view was quantified. (200× magnification). (c) FITC assay was used to measure intestinal permeability. (d) The positive hybridizing signal of total bacteria detected by EUB338 probe. EUB338 counts in intestinal epithelium (200× magnification) or pancreas (100× magnification) per field were quantified. Data are provided as the mean ± SEM (n = 6 per group). *means p< .05 vs WT, #means p< .05 vs AP.