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. 2022 Aug 18;14(1):2112882. doi: 10.1080/19490976.2022.2112882

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — L. reuteri protects mice from AP-induced ileal injury by inducing Paneth cells and maintaining intestinal stem cell number.

(a) Representative images of intestinal Paneth cells stained with lysozyme by immunofluorescence (200× magnification). (b-d) Intestinal mRNA expression of Lyz1, defa 5 and Nod2 in mice gut. (e) Representative images of intestinal stem cells stained with PCNA by immunofluorescence (200× magnification). *means p< .05 vs AP, #means p< .05 vs TKAP. (f) Representative images of Paneth cells (lysozyme+) and stem cells (PCNA+) in enteroids by immunofluorescence (400× magnification). (g) mRNA expression of Lyz1, defa 5, Lgr5 and Nod2 in enteroids with or without Lactobacillus incubation. Data are provided as the mean ± SEM (n = 6 per group). *means p< .05 vs Vehicle.