Figure 3.
HDM-challenged atg5−/− obese mice were refractory to steroid treatment. (A) Experimental timeline. atg5−/− obese mice and WT obese mice were immunized as described in Figure 1A. The mice were intraperitoneally treated with dexamethasone (1 mg/kg) 1 day before or after HDM challenges. (B) Lung resistance (RL) and dynamic compliance (Cdyn) (C) Differential cell counts in BALF. TCC, total cell count; MAC, macrophages; Ly, lymphocytes; Eo, eosinophils; Neut, neutrophils. (D) Hematoxylin and eosin staining. Original magnification: 100 × . (E) PAS staining. Data are representative of two independent experiments (n = 5–7/group). Data are expressed as the mean ± SEM. P-values were calculated using two-way ANOVA test post hoc test with the Tukey’s multiple comparison test.