Figure 3.

DCN mediates inflammation caused by ferroptotic cells. (A) Ferroptotic cells lacking Dcn, Atg5, or Mcoln1 do not elicit the production of the pro-inflammatory TNF and IL6 cytokine by 1 × 10⁶ BMDMs (n = 3 biologically independent samples; *P < 0.05, two-way ANOVA with Tukey’s multiple comparisons test; data are presented as means ± SD). (B,C) Anti-DCN neutralizing antibody (1 mg/ml) inhibits ferroptotic cells, but not late apoptotic cell- or necroptotic cell-induced TNF and IL6 release in BMDMs (n = 3 biologically independent samples; *P < 0.05, two-way ANOVA with Tukey’s multiple comparisons test; data are presented as means ± SD). (D,E) Lack of Dcn in late apoptotic or necroptotic cells fails to affect the production of TNF and IL6 by BMDMs (n = 3 biologically independent samples; data are presented as means ± SD).