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. 2022 Jan 24;18(9):2020–2035. doi: 10.1080/15548627.2022.2025572

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Figure 3. — Chemically defined LC3B–PE reconstitution systems. (A) LC3B–PE reconstitution system based on maleimide-thiol coupling. PE with a maleimide moiety in the head group (PE-maleimide) is incorporated into liposomes. LC3B^G120C directly interactes with PE-maleimide via a thiol-Michael addition reaction. (B) LC3B–PE reconstitution system based on a polyHis-NTA strategy. The PE mimic lipid DGS-NTA is included in the lipid composition used for preparing liposomes. DGS-NTA coordinates with His12-tagged LC3B to produce the LC3B-DGS noncovalent complex.