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. 2022 Jun 28;13(4):2132–2145. doi: 10.1002/jcsm.13026

Figure 1.

Figure 1

Expression of K320E‐Twinkle in skeletal muscle accelerates the accumulation of mtDNA alterations and mitochondrial dysfunction. (A) Long‐range PCR analysis showing multiple species of altered mtDNA in soleus (SOL) of 24‐month‐old K320Eskm mice. (B) qPCR analysis of three deletions (sum of Del 1, 3 and 17) in SOL, extensor digitorum longus (EDL) and gastrocnemius (GAS) muscles at 24 months of age. (C) qPCR analysis of K320Eskm GAS, showing ageing‐dependent increase in deletion levels (sum of Del 1, 3 and 17). (D) Representative cross sections, showing COX‐deficient (COX− and COX− /SDH++ (white *) fibres in various hindlimb muscles at 24 months (TA, tibialis anterior). (E–H) COX‐deficient fibre quantification showing accelerated and age‐dependent increase of mitochondrial dysfunction. n = 3–6 mice/group were used for deletions analyses and n = 6–10 mice/group to quantify the proportion of COX− fibres. Data are expressed as mean ± SEM, ***P < 0.001. Scale bars, 100 μm.