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. Author manuscript; available in PMC: 2022 Aug 23.

Published in final edited form as: J Autoimmun. 2022 Jul 8;131:102860. doi: 10.1016/j.jaut.2022.102860

Figure 3. — Characteristics of BCR repertoire from anti-dsDNA IgG+ B cells from spleen. C57/B6 mice were treated with S. aureus or B. subtilis PGNs via i.p. injection twice per week for 8 weeks. At the end of study, B cells from mice at 14 weeks of age were isolated from spleen and anti-dsDNA IgG+ B cells were sorted using flow cytometry. RNA was extracted, and BCR sequencing was conducted. BCR clonality was analyzed in C57/B6 mice treated with S. aureus (SA) and B. subtilis (BS) PGN (n = 4 per group). (A) The frequency of IGHV usage. (B) BCR clonal diversity. (C) The frequency of CDR3 length. (D) Somatic mutation of IGHV3-74 and IGHV1-45. Statistical analysis used non-parametric Mann-Whitney U tests.

Figure 3. — Characteristics of BCR repertoire from anti-dsDNA IgG+ B cells from spleen. C57/B6 mice were treated with S. aureus or B. subtilis PGNs via i.p. injection twice per week for 8 weeks. At the end of study, B cells from mice at 14 weeks of age were isolated from spleen and anti-dsDNA IgG+ B cells were sorted using flow cytometry. RNA was extracted, and BCR sequencing was conducted. BCR clonality was analyzed in C57/B6 mice treated with S. aureus (SA) and B. subtilis (BS) PGN (n = 4 per group). (A) The frequency of IGHV usage. (B) BCR clonal diversity. (C) The frequency of CDR3 length. (D) Somatic mutation of IGHV3-74 and IGHV1-45. Statistical analysis used non-parametric Mann-Whitney U tests.