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Diagram of the different probes (within the ptxR-ptxS intergenic region) that were used in the gel shift assays. The 304- and 103-bp fragments of the ptxS upstream region were generated by restriction digestion using available restriction sites (B, BamHI; D, DpnI; H, HincII; K, KpnI). The 52- and 63-bp fragments were synthesized by PCR. Arrows indicate the direction of transcription of ptxR and ptxS.
