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Autoregulation of ptxS expression in P. aeruginosa. Plasmid pBS8-4 (which carries a ptxS-lacZ fusion) was introduced into PAO1 and PAO1::ptxS. Cells were grown in TSB-DC medium for 14 h, and the level of β-galactosidase activity was determined as previously described (16). PAO1 carrying pSW205 (negative control) produced no β-galactosidase activity. The values are averages of three independent experiments ± the standard error of the mean.
