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. 2021 Sep 27;81(23):5889–5903. doi: 10.1158/0008-5472.CAN-21-1538

Figure 2.

Figure 2. PLCB1 is important for the proliferation and oncogenicity of CCA cells. A, PLCB1 overexpression and knockdown could affect proliferation abilities of indicated CCA cell lines via Edu assay analysis. **, P < 0.01; ***, P < 0.001, based on Student t test. Scale bars, 100× = 400 μm. B, Typical images of colony formation assays and colony counts. *, P < 0.1; **, P < 0.01; ***, P < 0.001, based on Student t test. C, Typical images of subcutaneous xenograft mice models and hematoxylin and eosin staining. Scale bars, 400× = 100 μm. D, Typical images of bioluminescence pictures, hematoxylin and eosin (H&E), and CK19 staining of vector/sgPTEN/sgP53 and PLCB1/sgPTEN/sgP53 C57BL mice livers at 8 weeks after hydrodynamic injection respectively. ****, P < 0.0001, based on Student t test. Scale bars, 100× = 400 μm. All experiments were implemented three times, and data are presented as mean ± SD.

PLCB1 is important for the proliferation and oncogenicity of CCA cells. A, PLCB1 overexpression and knockdown could affect proliferation abilities of indicated CCA cell lines via Edu assay analysis. **, P < 0.01; ***, P < 0.001, based on Student t test. Scale bars, 100× = 400 μm. B, Typical images of colony formation assays and colony counts. *, P < 0.1; **, P < 0.01; ***, P < 0.001, based on Student t test. C, Typical images of subcutaneous xenograft mice models and hematoxylin and eosin staining. Scale bars, 400× = 100 μm. D, Typical images of bioluminescence pictures, hematoxylin and eosin (H&E), and CK19 staining of vector/sgPTEN/sgP53 and PLCB1/sgPTEN/sgP53 C57BL mice livers at 8 weeks after hydrodynamic injection respectively. ****, P < 0.0001, based on Student t test. Scale bars, 100× = 400 μm. All experiments were implemented three times, and data are presented as mean ± SD.