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. 2021 Sep 27;81(23):5889–5903. doi: 10.1158/0008-5472.CAN-21-1538

Figure 6.

Figure 6. PABPC1 is closely related to PLCB1-mediated PI3K/AKT/GSK3β/snail signaling in CCA. A, Endogenous and exogenous coimmunoprecipitation indicated that PLCB1 could interact with PABPC1. B, Colocalization between PLCB1 and PABPC1 was explored by immunofluorescence in the indicated CCA cell lines. Scale bars, 400× = 100 μm. C, Typical images of the Duolink PLA experiment predicted PABPC1 directly interacted with PLCB1 via the fluorescence microscope. Scale bars, 400× = 100 μm. D and E, The expression levels of key proteins of the PI3K/AKT pathway and EMT process for indicated CCA cell lines were determined by Western blot after stable PABPC1 knockdown and overexpression lentiviral transfection. All experiments were implemented three times, and data are presented as mean ± SD.

PABPC1 is closely related to PLCB1-mediated PI3K/AKT/GSK3β/snail signaling in CCA. A, Endogenous and exogenous coimmunoprecipitation indicated that PLCB1 could interact with PABPC1. B, Colocalization between PLCB1 and PABPC1 was explored by immunofluorescence in the indicated CCA cell lines. Scale bars, 400× = 100 μm. C, Typical images of the Duolink PLA experiment predicted PABPC1 directly interacted with PLCB1 via the fluorescence microscope. Scale bars, 400× = 100 μm. D and E, The expression levels of key proteins of the PI3K/AKT pathway and EMT process for indicated CCA cell lines were determined by Western blot after stable PABPC1 knockdown and overexpression lentiviral transfection. All experiments were implemented three times, and data are presented as mean ± SD.