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. 2021 Nov 22;82(2):264–277. doi: 10.1158/0008-5472.CAN-21-1171

Figure 1.

Figure 1. Elevated BAFF in the TME slows tumor growth. A, C57BL/6 mice were inoculated intravenously with 105 of BAFF-expressing or control cells (n = 6–7, pooled from two independent in vivo experiments). B and C, Tumor growth of 5 × 105 subcutaneously injected BAFF-expressing and control cells in C57BL/6 mice are shown (n = 5–6; B), as are tumor weights (n = 8–10, pooled from at least two independent in vivo experiments; C). D, Gene expression levels of BAFF (Tnfsf13B) were determined in whole tumors. Expression was normalized to Gapdh (n = 5–12, pooled from at least two independent in vivo experiments). E, BAFF protein expression was confirmed using immunohistochemical staining of tumor tissue (representative images of n = 5–12 mice pooled from at least two independent in vivo experiments are shown). Scale bar, 50 μm. Error bars in the all experiments indicate SEM. *, P < 0.05 as determined by a Student t test (unpaired, two-tailed) and log-rank test for analysis of Kaplan–Meier survival curves.

Elevated BAFF in the TME slows tumor growth. A, C57BL/6 mice were inoculated intravenously with 105 of BAFF-expressing or control cells (n = 6–7, pooled from two independent in vivo experiments). B and C, Tumor growth of 5 × 105 subcutaneously injected BAFF-expressing and control cells in C57BL/6 mice are shown (n = 5–6; B), as are tumor weights (n = 8–10, pooled from at least two independent in vivo experiments; C). D, Gene expression levels of BAFF (Tnfsf13B) were determined in whole tumors. Expression was normalized to Gapdh (n = 5–12, pooled from at least two independent in vivo experiments). E, BAFF protein expression was confirmed using immunohistochemical staining of tumor tissue (representative images of n = 5–12 mice pooled from at least two independent in vivo experiments are shown). Scale bar, 50 μm. Error bars in the all experiments indicate SEM. *, P < 0.05 as determined by a Student t test (unpaired, two-tailed) and log-rank test for analysis of Kaplan–Meier survival curves.