Table 1.

Potential tumorigenicity-associated risk factors in hPSCs-based therapy and applicable methodologies for their detection or prevention.

Potential risk factor for tumor formation	Applicable detection or prevention methods
Large to medium chromosomal aberrations	G-banding, spectral karyotype (SKY), Array-CGH, e-Karyotype, WES.
Small copy-number variation	SNP-array, quantitative-PCR, FISH, eSNP-karyotype, WES
Pathogenic point mutations	Sanger sequencing, RNA-seq, WES, WGS
Cell line specific tumorigenic or malignant propensity	Teratoma assay
Residual hPSCs in the transplant	Chemical ablation, immunological targeting, genetic elimination