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. 2022 Jun 18;11(8):861–875. doi: 10.1093/stcltm/szac037

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — sEV characterization by both NTA (data from 6 technical repeats) and Cryo-EM demonstrated particles of the expected size for sEVs for both OMLP-PC_L (A&C) and MSCs (B&D; Scale bar = 100 nm). Tetraspannin expression was determined by flow cytometry and demonstrated OMLP-PC_L sEVs (E) and MSC sEVS (F) were positive for CD9, CD81, and CD63. Fluorescently labeled sEVs (Green-488) from OMLP-PC_L (G) and MSCs (H) demonstrated uptake into dermal fibroblasts which were counterstained to visualize actin (Magenta) and the cell nucleus (Blue)—images are confocal slices. Scale bar = 50 μm. Error bars for NTA = SD.