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. 2022 Jun 27;11(8):876–888. doi: 10.1093/stcltm/szac042

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 7. — Scleraxis expressing cells mark the areas that give rise to the heterotopic bone. Scleraxis (Scx)-GFP reporter animals underwent induction of heterotopic ossification (HO) by acetabular reaming, followed by analysis at 3 weeks post-operatively. Scx-GFP+ cells appear green. (A) Axial section taken from an uninjured hip (A-a’) with high magnification pictures showing more in detail the soft tissue (a) and periosteal (a’) areas. (B-b’) Axial section obtained 3 weeks after HO induction, demonstrating heterotopic bone in the soft tissue and periosteal periacetabular areas (b-b’). Abbreviations: p, periosteum area; t, tendon; ar, acetabular roof; ib, iliac bone; rt, reactive tissue; cb, cortical bone. DAPI nuclear counterstain appears blue. Yellow scale bar: 100 μm. Red scale bars: 20 μm.