FIG. 2.

Purification of recombinant Pur7. A Coomassie-stained SDS–11% polyacrylamide gel is shown. Lanes: 1, crude extract of E. coli BL21(DE3)pLysS(pRSETb); 2, crude extract of E. coli BL21(DE3)pLysS(pUR7.EX) 2 h after addition of IPTG; 3, first eluate from the Ni²⁺-NTA agarose column; 4, eluate with sodium phosphate buffer; 5, eluate with 20 mM imidazole-containing buffer; 6, eluate with 500 mM imidazole-containing buffer; M, molecular mass markers (sizes are in kilodaltons). The arrow indicates recombinant Pur7.