TABLE 2.

Substrate specificity of recombinant Pur7

Substratea	Specific activity (U/mg of protein)
3′-Amino-3′-dATP	54.6
3′-Amino-3′-dTTP	20.2
2′-Amino-dATP	<0.1
8-Oxo-dGTP	<0.1
dPTP	<0.1
3′-Azido-3′-dTTP	<0.1
3′-Fluoro-3′-dTTP	<0.1
3′-Deoxy-ATP (cordycepin triphosphate)	<0.1
Nucleoside triphosphates	<0.1
2′-Deoxy-nucleoside triphosphates	<0.1

^aSubstrate concentrations were 2 mM except in the cases of 3′-amino-3′-dATP and 3′-amino-3′-dTTP, for which concentrations were 1 mM.