Fig 3. RBD expression and purification.
(A) Design of the expression cassette integrated into the recombinant baculovirus. (B) Detection of RBD from infected culture supernatants using an anti-His (left) and anti-spike (right) antibody. Bv-WT: Wild type baculovirus; Bv-RBD: RBD expressing baculovirus. (C) SDS-PAGE of purified RBD after the affinity chromatography purification step (Lane 1) and size exclusion chromatography (Lane 2).