Fig 5. Detection of specific antibodies against RBD in mice.
Immunized mice were bled at 0, 15, 30 and 45 days post immunization. All sera were obtained by low-speed centrifugation. Serum samples were processed to detect specific antibodies against SARS-CoV-2 RBD protein using indirect ELISA assay. (A) Group immunized with 20 μg of RBD mixed with A1 and A3 (B) Group immunized with RBD 50 μg of RBD mixed with A1 and A4. Two-way ANOVA and post-hoc Tukey’s test were performed. *: P<0.01.