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. 2022 Aug 23;17(8):e0269823. doi: 10.1371/journal.pone.0269823

Fig 7. Evaluation of cellular immunity in mice vaccinated with purified RBD.

Fig 7

Mice were immunized with 20 and 50 μg of RBD using two different adjuvants (A1 and A3) at 0, 15 and 30 days post immunization. On day 45 post-immunization mice were euthanized and spleens were processed. (A) Percentage of CD4 and CD8 positive cells by flow cytometry, between the groups immunized (n = 3, except the adjuvant control). (B) IFN-γ ELISPOT of splenocytes between the groups immunized (n = 3, except the adjuvant control). (C) Intracellular staining of Th1 cytokines (IFN-γ, TNF-α and IL-2) of splenocytes stimulated with RBD (n = 3, except the adjuvant control). ns: not significant (P>0.05), *: P<0.05.