Skip to main content
. 2022 Aug 11;18(8):e1010169. doi: 10.1371/journal.pgen.1010169

Fig 1. 2DG treatment induces the endocytosis of many plasma membrane proteins, that is mostly Rod1-dependent.

Fig 1

(A) Cells expressing Hxt2-GFP and Tat1-GFP cells were grown overnight in a glucose-containing medium (exponential phase) and treated with 2DG for 2h30 and observed by fluorescence microscopy. PM: plasma membrane; V: vacuole. Scale bar: 5 μm. (B) Western blot on total protein extracts from Hxt2-GFP- and Tat1-GFP-expressing cells before and after 2DG addition for 2h30, using anti-GFP antibodies. (C) Plasma membrane proteins whose localization was examined by fluorescence microscopy before and after 2h30 2DG. When endocytosed, the same proteins were also observed in a rod1Δ context to check for Rod1 dependence (NT, not tested). (D) Fluorescence microscopy of WT and rod1Δ cells expressing the indicated transporters tagged with GFP before and after 2DG treatment for 4h. Scale bar: 5 μm.