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. 2022 Aug 11;18(8):e1010169. doi: 10.1371/journal.pgen.1010169

Fig 3. 2DG causes a fast and temporary dephosphorylation of Snf1 and its substrates through PP1 activation.

Fig 3

(A) Total protein extracts of WT, reg1Δ and snf1Δ cells were prepared before and after 2DG addition at the indicated concentrations for 10 min and immunoblotted using anti-phospho-AMPK and anti-polyHistidine antibodies. (B) Left, Total protein extracts of WT, reg1Δ and snf1Δ cells were prepared before and after 2DG addition for the indicated time and immunoblotted using anti-phospho-AMPK and anti-polyHistidine antibodies. Right, quantification of the signals (adjusted p-value is indicated, one-way ANOVA). (C) Total protein extracts of WT and reg1Δ cells expressing Snf1-GFP and Rod1-Flag were prepared before and after 2DG addition for the indicated time and immunoblotted using anti-Flag, anti-phospho-AMPK and anti-GFP antibodies. (D) Total protein extracts of WT, reg1Δ and snf1Δ cells expressing Mig1-Flag were prepared before or after 2DG addition for 10’ and 2h and immunoblotted using anti-Flag antibodies.