Fig 5. Low affinity glucose transporters are required for the resistance of the rod1Δ mutant to 2DG.

(A) Beta-galactosidase assays of WT and rod1Δ cells expressing LacZ under the control of the pDOG1 or pDOG2 promoters, before and after 2DG treatments for 4h (+- SEM, n = 3 independent experiments, t-test). A.U., arbitrary units. (B-D) Serial dilutions of cultures of the indicated strains were spotted on SC medium or SC + 0.2% 2DG medium and grown for 4 days at 30°C.