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Most antibodies used in immunoassays target the C-terminal post-translationally modified region of αSYN. The main αSYN PTMs (phosphorylation, nitration, and truncation) are depicted. Red and green bars above the sequence of αSYN show the epitopes of capture and detection antibodies, respectively, that have been used in immunoassays to measure total αSYN, pS129 αSYN, and aggregated αSYN levels in human biological specimens.
