Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 8;12(5):1423–1447. doi: 10.3233/JPD-213128

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 – The authors. Published by IOS Press

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 8 — PLA of pS1292-LRRK2 detection in rat brain injected with HdAd5 vectors (thymidine kinase, WT LRRK2 and G2019S LRRK2) at 42 days post-injection. A) PLA with pS1292 LRRK2 antibody and total LRRK2 antibody (N241A/34) in rat SNpc. Sections were pre-immunostained with antibody to tyrosine hydroxylase (TH) following with corresponding fluorescent secondary antibody. Images taken with confocal microscope at 63x magnification showing PLA signal (red), TH signal (magenta) and DAPI (blue) for ipsilateral SNpc. PLA controls (-PLUS) include samples incubated without PLUS probe. PLA pS1292-LRRK2 (BOTH) include samples incubated with both PLUS and MINUS probes. Scale bar, 50 μm. B) PLA with pS1292-LRRK2 antibody and total LRRK2 antibody (N241A/34) followed by post-PLA labeling with FLAG antibody in rat SNpc. Sections were pre-immunostained with antibody to TH and following with corresponding fluorescent secondary antibody. After terminating the pS1292-LRRK2 PLA reaction, sections were probed with antibody to FLAG following with corresponding fluorescent secondary antibody. Images taken with confocal microscope at 63x magnification showing PLA S1292-LRRK2 signal (red), TH signal (magenta), FLAG signal (green) and DAPI (blue) for ipsilateral SNpc. Yellow arrowheads indicate FLAG-positive cells in ipsilateral SNpc and their corresponding PLA pS1292-LRRK2 and TH signals. White arrowheads indicate FLAG-negative cells in ipsilateral SNpc and their corresponding PLA pS1292-LRRK2 and TH signals. PLA controls (-PLUS) include samples incubated without PLUS probe. PLA pS1292-LRRK2 (BOTH) include samples incubated with both PLUS and MINUS probes. Scale bar, 50 μm.