FIG. 7.

Identification of other target sites in P. atlantica DB27. (A) Agarose gel electrophoresis of PCR products generated from inverse PCR (described in Materials and Methods) of diluted and ligated P. atlantica DB27 chromosomal DNA following digestion with either RsaI (lanes 1 to 3) or HaeIII (lanes 4 to 6). Either 0.25 μg (lanes 1 and 4), 0.025 μg (lanes 2 and 5), or 0.0005 μg (lanes 3 and 6) of P. atlantica chromosomal DNA was used as a template in inverse PCRs. Lane M, Promega 1-kb marker. The inverse image of the UV-illuminated ethidium bromide-stained gel is shown. (B) Alignment of target site sequences. The duplicated target sequence is indicated with a box.