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. 2022 Aug 9;15:896183. doi: 10.3389/fnmol.2022.896183

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Copyright © 2022 Ayajuddin, Phom, Koza, Modi, Das, Chaurasia, Thepa, Jamir, Neikha and Yenisetti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dose and time-dependent mortality of Drosophila (OK) exposed to rotenone during different phases of adult life. Mortality pattern among adult male flies of early health span (A), late health span (B), and transition phase (C), exposed to seven different concentrations of rotenone (10, 25, 50, 100, 250, 500, and 1,000 μM) showed concentration-dependent lethality. Mortality data were collected every 24 h for each group till all the flies were dead. There was no mortality up to the 6th day for health span flies and the 3rd day for late health and transition phase flies. Comparison of survival curves reveals that the response difference among different tested concentrations was significant (log-rank [Mantel–Cox] test. p < 0.0001).