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. 2022 Aug 9;15:896183. doi: 10.3389/fnmol.2022.896183

FIGURE 2.

FIGURE 2

Assessing the climbing ability of Drosophila as determined by negative geotaxis assay after exposure to multiple concentrations of rotenone (10, 25, 50, 100, 250, 500, and 1,000 μM). About 500 μM of rotenone-induced clear mobility defects in the adult male fly of early health span on 5th day (A), 25 μM rotenone in late health span fly on the 2nd day (B) and 10 μM rotenone in transition phase fly on the 2nd day (C) and there was no mortality at the selected time points. Observation of mere mobility defects but not mortality of fly is the reason behind selecting above mentioned toxin concentrations and durations of toxin exposure in adult life stage-specific fashion. Hence, these concentrations of toxin and window period of exposure were selected for further studies. Data were collected every 24 h for each group. One-way ANOVA followed by the Newman–Keuls Multiple Comparison Test showed a significant difference in mobility. *p < 0.05, ***p < 0.0001, NS, Not significant.

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