TABLE 1.
Bacterial strains and plasmids used in this work
| Strain | Genotype | Reference or source |
|---|---|---|
| S. typhimurium | ||
| SL1344 | Wild type | 19 |
| SVM473 | Cmr Φ(sigD-lacZYA)/sigDE+ | This work |
| SB154 | Cmr StrRinvA::Ωcat | J. Galán |
| SVM514 | Strr SprspaS::ΩStr/Sp | This work |
| SVM579 | ΔinvF (in-frame deletion of 465 bp) | This work |
| VV302 | ΔhilA-523 | 3 |
| BJ68 | Tcr; sipC::Tn5lacZY | 34 |
| SVM725 | TcrsipC::Tn5lacZY ΔinvF | This work |
| SVM733 | Strr SprspaS::ΩStr/Sp ΔinvF | This work |
| SVM754 | Strr Spr Tcr ΔinvF spaS::ΩStr/Sp sipC::Tn5lacZY | This work |
| LB5000 | LT2, flaA66 metA22 trp-2 rpsL xyl-401 ilv-452 leu hsd mod+ | 37 |
| Plasmids | ||
| pHG329 | Apr medium-copy-number cloning vector | 39 |
| pWKS130 | Knr, low-copy-number cloning vector | 40 |
| pVV214 | Apr, hilA in pACYC177 | 3 |
| philA | Knr, 2.2-kb NsiI fragment from pVV214 cloned into the PstI site of pWSK130 | This work |
| pHH21 | Apr, sigD-lacZYA, 0.9-kb EcoRI/PstI sigDE promoter and partial coding sequences in pRS415 | 20 |
| pVLT33 | Knr, low-copy-vector for IPTG-inducible expression downstream of Ptac | 9 |
| pHH37 | Knr, 3.2-kb EcoRI-BamHI sigDE fragment in pVLT33 | This work |
| pRW50 | Tcr, low-copy-number transcriptional reporter fusion vector | 29 |
| pHD3 | Tcr, Φ(invF-lacZYA) in pRW50 | This work |
| pHD11 | Tcr, Φ(sicA-lacZYA) in pRW50 | This work |
| pFUSE | Cmr, suicide vector for integration of lacZYA transcriptional reporters onto the chromosome | 5 |
| pHD5 | Cmr, 1-kb sequence including sigDE promoter in pFUSE | This work |
| pDL7-2 | Tcr, cosmid clone containing inv-spa and sicAB genes | 32 |
| pHD7 | Knr, ∼8-kb EcoRI fragment from pDL7-2 containing invHFGEABC′ in pWKS130 | This work |
| pHD8 | Knr, ∼6.7-kb EcoRI fragment from pDL7-2 containing invC′IJ spaOPQRS sicA sequence in pWSK130 | This work |
| pHD9-1 | Knr, 1.7-kb PstI fragment from pHD7 containing invF in pWKS130 | This work |
| pHD10-1 | Apr, 1.7-kb PstI fragment from pHD7 containing invF in pHG329 | This work |
| pHD17 | Cmr, 1.7-kb HindIII invF fragment from pHD10-1 cloned into pACYC184 | This work |
| pHD13 | Apr, same as pHD10-1 but with 465-bp deletion in invF | This work |
| pMAK705 | Cmr, temperature-sensitive origin of replication vector used for gene replacement | 16 |
| pHD15 | Cmr, ∼1.25-kb PstI fragment from pHD13 cloned into pMAK705 | This work |