TABLE 4.
Complementation of the ΔinvF mutant for invasion of HEp-2 cells
| Strain | Mean % invasion ± SDa (% of wild-type level)
|
|||
|---|---|---|---|---|
| No plasmid | pWKS130 (vector) | pHD9 (invF+) | philA | |
| SL1344 | 12.4 ± 0.3 (100) | 7.7 ± 1.4 (100) | 7.1 ± 1.3 (100) | 8.0 ± 2.0 (100) |
| SVM579 (ΔinvF) | 0.6 ± 0.1 (5) | 0.6 ± 0.1 (7) | 6.8 ± 1.3 (96) | 3.2 ± 0.3 (40) |
| SB154 (invA::Ωcat) | 0.02 ± 0.006 (0.1) | ND | ND | ND |
| SVM514 (spaS::ΩStr/Sp) | 0.02 ± 0.001 (0.1) | ND | ND | ND |
a
After bacteria were allowed to invade during a 1-h incubation. Values are representative of several assays done in duplicate. ND, not determined.