FIG. 1.

Relative β-galactosidase levels of pucE′::lac′Z fusions with different amounts of puc 5′ sequences. The β-galactosidase specific activities of SB1003 strains containing each plasmid were normalized to that of SB1003(pPEZ), and standard deviations are shown in parentheses. Genes are indicated by boxes, and the inverted repeat between pucA and pucC, if present, is shown as a stem and loop. The extra nucleotides resulting from filling in of a BclI site early in the pucD open reading frame to create a stop codon are indicated by triangles above the appropriate constructs.