Appendix 1—table 2. Examples of SerialEM settings for SmartScope usage.
These settings serve as guidelines and will need to be adapted for different hardware combinations. Updated versions of this table can be found at github.com/NIEHS/SmartScope.
| Example 1 | Example 2 | |
|---|---|---|
| Instrument | ||
| Microscope | Talos Arctica | Titan Krios G4 |
| Detector | Gatan K2 Summit | Gatan K3 |
| Energy filter | - | Gatan BioContiuum |
| Low Dose Presets | ||
| Search | ||
| Magnification | 210 | 580 |
| Pixel size (Å/pixel) | 196 | 152 |
| Mode | Linear | Counting |
| View | ||
| Magnification | 2600 | 8700 |
| Pixel size (Å/pixel) | 16.1 | 10.1 |
| Mode | Linear | Counting |
| Focus/record | ||
| Magnification* | 36,000 | 81,000 |
| Pixel size (Å/pixel)* | 1.19 | 1.08 |
| Mode | Counting | Counting |
| Full grid montage presets | ||
| Magnification | 62 | 135 |
| Pixel size (Å/pix) | 644 | 654 |
| Mode | Linear | Counting |
*
These are the presets that are used for screening. They can be changes to suit the requirement for the specimen.