Table 1. Cryo-EM data acquisition parameters and statistics.
| POLG2 (EMD-25764) | |||
|---|---|---|---|
| No tilt | Tilted | Combined | |
| Hardware | |||
| Microscope | Talos Arctica (Thermo Fisher) | ||
| Detector | K2 summit (Gatan Inc) | ||
| Data collection and processing | |||
| Magnification | 45,000 | ||
| Voltage (kV) | 200 | ||
| Electron exposure (e-/Å2) | 50 | ||
| Defocus range (µm) | 1.2–1.8 | 1.4–1.6 | 1.2–1.8 |
| Tilt angle (°) | 0 | 30 | |
| Pixel size (Å/pixel) | 0.932 | ||
| Movie No. | 3029 (70%) | 1282 (30%) | 4311 |
| Symmetry imposed | C2 | ||
| Final particles | 99,189 (78%) | 28,641 (22%) | 127,860 |
| Map resolution (unmasked) | 3.7 | 4.3 | 3.7 |
| Map resolution (masked) | 3.5 | 3.9 | 3.4 |
| FSC threshold | 0.143 | ||
| Data collection statistics | |||
| Setup time* (min) | 60 | ||
| Throughput (movies/hr) | 117.9 | ||
| Throughput last hour (movies) | 130 | ||
*
Data collection times are calculated as the time needed from grid loading to the collection of the first 50 high-magnification targets minus the time required for these 50 targets to be acquired.