Figure 4. Use Case 2: Mice wearing Miniscopes.

(A) Design for MiniCAM, an open-source camera designed to interface with Miniscopes and pose tracking. (B) Still frame from MiniCAM recording of mouse wearing a V4 Minscope. DLC tracked points are labeled with rainbow dots. Scale bar, 2 cm. (C) Performance of freezing heuristic on videos of mouse wearing Miniscope recorded with MiniCAM (Precision: 0.85; Recall: 0.93; F1 Score: 0.89; Specificity: 0.84). (D) Task design. (E) Sample BehaviorDEPOT output for mouse wearing Miniscope during PMA. Map displays animal position over time as well as freezing locations (black squares). (F) Summary behavioral data for training. (G) Summary behavioral data for retrieval. (H) GCaMP7-exressing mPFC neurons imaged through a V4 Miniscope. Scale bars, 100 μm. (I) Example Ca²⁺ traces from platform (top) and tone (bottom) modulated cells during time on the platform (blue) or time freezing (pink). (J) Receiver operating characteristic (ROC) curves that were calculated for platform-modulated cells (excited cell: auc = 0.71; suppressed cell: auc = 0.35, unmodulated cell: auc = 0.45) and freezing-modulated cells (excited cell: auc = 0.81; suppressed cell: auc = 0.32; unmodulated cell: auc = 0.42). (K) Example field of view showing locations of freezing- and platform-modulated mPFC neurons. (L) Proportion of modulated cells of each functional type from 513 cells recorded across 3 mice. Error bars represent S.E.M.