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Southern analysis of antibiotic-resistant H. influenzae isolates. (A) Genomic Southern blot of trimethoprim-resistant colonies. (B) Genomic Southern blot of chloramphenicol-resistant colonies. Lanes: 1 to 24, 1 colony/lane; 25 to 30, three colonies/lane. (A) Lanes 1 to 31, EcoRI digest; 31 to 36, EcoRI-BamHI double digest. (B) Lanes 1 to 31, EcoRI digest; +, positive controls for Southern hybridization with AT-2 and AT-Cm, respectively.
