Figure 10.
BACE2 Aβ cleaved fragments do not have increased abundance in the young cortex of the Dp3Tyb;AppNL-F/NL-F model. Liquid chromatography-MS analysis of immunoprecipitated cortical Aβ from FA fraction normalized to weight of cortical tissue was used to determine whether the Dp3Tyb region was sufficient to alter the abundance of putative BACE2 cleavage products at 3 months of age. A, Dp3Tyb;AppNL-F/NL-F cortex weighs more than AppNL-F/NL-F cortex at 3 months of age (F(1,22) = 7.772, p = 0.011). AppNL-F/NL-F (female n = 8, male n = 5), Dp3Tyb;AppNL-F/NL-F (female n = 4, male n = 9). No significant increase in the abundance of (B) Aβ1-19, (F(1,20) = 0.166 p = 0.688), (C) Aβ1-20 (F(1,20) = 0.274 p = 0.607), or (D) Aβ1-34 (F(1,20) = 0.005 p = 0.942) was observed. No difference in the abundance of (E) Aβ1-14, (F(1,15) = 1.622, p = 0.222), (F) Aβ1-15, (F(1,19) = 0.496, p = 0.490), (G) Aβ1-16, (F(1,19) = 2.274, p = 0.148), or (H) Aβ1-17, (F(1,19) = 0.079, p = 0.781) was detected in the cortex of Dp3Tyb;AppNL-F/NL-F compared with AppNL-F/NL-F mice. AppNL-F/NL-F (female n = 8, male n = 5), Dp3Tyb;AppNL-F/NL-F (female n = 4, male n = 9). For clarity: Dp3, Dp3Tyb. Error bars indicate SEM, *p < 0.05. Data points represent independent mice. For Aβ1-19 and Aβ1-20, n = 1 sample was below the limit of detection per genotype. Aβ1-14, n = 3 samples were below the limit of detection per genotype, Aβ1-15, 1-16, 1-17, n = 1 samples were below the limit of detection per genotype. These samples are shown on the graphs but were excluded from ANOVA.