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. 2022 Aug 17;42(33):6453–6468. doi: 10.1523/JNEUROSCI.0521-22.2022

Figure 5.

Figure 5.

Biochemical solubility of Aβ42 is not altered by an additional copy of the Dp3Tyb, Dp(10)2Yey, or Dp(17)3Yey Hsa21 orthologous regions. Total cortical proteins were biochemically fractionated from 8-month-old mice and Aβ abundance analyzed by MSD assay (6E10). No difference in the abundance of (A) 5 m guanidine hydrochloride-soluble Aβ42 (F(1,12) = 0.128, p = 0.726), (D) 1% Triton X-100-soluble Aβ42 (F(1,12) = 2.863, p = 0.116), or (G) Tris-soluble Aβ42 (F(1,10) = 0.281, p = 0.608) was observed between Dp3Tyb;AppNL-F/NL-F compared with AppNL-F/NL-F controls. AppNL-F/NL-F female n = 2, male n = 6; Dp3Tyb;AppNL-F/NL-F female n = 7, male n = 3. No difference in the abundance of (B) 5 m guanidine hydrochloride-soluble (F(1,11) = 2.337, p = 0.115), (E) 1% Triton X-100-soluble Aβ42 (F(1,11) = 0.145, p = 0.711), or (H) Tris-soluble Aβ42 (F(1,11) = 0.001, p = 0.978) was observed between Dp(10)2Yey;AppNL-F/NL-F compared with AppNL-F/NL-F controls. AppNL-F/NL-F female n = 4, male n = 5; Dp(10)2Yey;AppNL-F/NL-F female n = 6, male n = 3. No difference in the abundance of (C) 5 m guanidine hydrochloride-soluble (F(1,20) = 4.079, p = 0.057), (F) 1% Triton X-100-soluble Aβ42 (F(1,20) = 0.124, p = 0.728), or (I) Tris-soluble (F(1,20) = 0.521, p = 0.479) Aβ42 was observed between Dp(17)3Yey;AppNL-F/NL-F compared with AppNL-F/NL-F controls. AppNL-F/NL-F female n = 7, male n = 5; Dp(17)3Yey;AppNL-F/NL-F female n = 7, male n = 7. Details of negative controls, which do not carry an AppNL-F allele in Table 2. For clarity: Dp3, Dp3Tyb; Dp10, Dp(10)2Yey; Dp17, Dp(17)3Yey. Error bars indicate SEM. Data points represent independent mice.