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. 2022 Aug 17;42(33):6453–6468. doi: 10.1523/JNEUROSCI.0521-22.2022

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Figure 6. — Biochemical solubility of Aβ₄₀ is not altered by an additional copy of the Dp(10)2Yey or Dp(17)3Yey Hsa21 orthologous regions. Total cortical proteins were biochemically fractionated from 8-month-old mice and Aβ abundance analyzed by MSD assay (6E10). A, No difference in the abundance of 5 m guanidine hydrochloride-soluble Aβ₄₀ was observed between Dp(10)2Yey;App^NL-F/NL-F compared with App^NL-F/NL-F controls (F_(1,12) = 2.137, p = 0.169). App^NL-F/NL-F female n = 6, male n = 3; Dp(10)2Yey;App^NL-F/NL-F female n = 4, male n = 5. B, No difference in the abundance of 5 m guanidine hydrochloride-soluble Aβ₄₀ was observed between Dp(17)3Yey;App^NL-F/NL-F compared with App^NL-F/NL-F controls (F_(1,1) = 0.782, p = 0.539). App^NL-F/NL-F female n = 1, male n = 2, n = 9 below limit of detection; Dp(17)3Yey;App^NL-F/NL-F female n = 2, male n = 0, n = 12 below limit of detection. C, No difference in the abundance of 1% Triton X-100-soluble Aβ₄₀ was observed between Dp(10)2Yey;App^NL-F/NL-F compared with App^NL-F/NL-F controls (F_(1,11) = 0.540, p = 0.478). App^NL-F/NL-F female n = 6, male n = 3; Dp(10)2Yey;App^NL-F/NL-F female n = 4, male n = 4. D, No difference in the abundance of 1% Triton X-100-soluble Aβ₄₀ was observed between Dp(17)3Yey;App^NL-F/NL-F compared with App^NL-F/NL-F controls (F_(1,19) = 0.007, p = 0.982). App^NL-F/NL-F female n = 7, male n = 5; Dp(17)3Yey;App^NL-F/NL-F female n = 7, male n = 6, n = 1 below limit of detection. For details of negative controls, which do not carry an App^NL-F allele, see Table 2. Error bars indicate SEM. Data points represent independent mice. For clarity: Dp10, Dp(10)2Yey; Dp17, Dp(17)3Yey.