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. 2022 Aug 17;42(33):6453–6468. doi: 10.1523/JNEUROSCI.0521-22.2022

Figure 8.

Figure 8.

The abundance of FL-APP and CTF is not altered by duplication of the Dp10Yey Hsa21 orthologous region. The abundance of (A,D) FL-APP, (B,D) C-terminal fragment-α (CTF-α) and (C,D) CTF-β relative to β-actin loading control was measured by western blot in the cortex at 3 months of age in male and female mice. There was no effect of an extra copy of the Dp(10)2Yey region on the abundance of (A) FL-APP level (F(1,22) = 0.828, p = 0.372), (B) CTF-α (F(1,22) = 0.054, p = 0.819), or (C) CTF-β abundance (F(1,22) = 0.829, p = 0.372). As previously observed, mice homozygous for the AppNL-F allele had lower abundance of (A) FL-APP (F(1,22) = 8.168, p = 0.009), (B) CTF-α (F(1,22) = 72.150, p < 0.001), and a higher abundance of (C) CTF-β (F(1,22) = 15.300, p < 0.001). WT (female n = 1, male n = 4), Dp(10)2Yey (male n = 8), AppNL-F (male n = 9), and Dp(10)2Yey;AppNL-F/NL-F (female n = 2, male n = 4). Error bars indicate SEM, **p < 0.01 and ***p < 0.001. Data points represent independent mice. For clarity: Dp10, Dp(10)2Yey.