Table 2.
Summary of negative control samples, which do not carry an AppNL-F allele and do not produce human Aβ, used in MSD assaysa
| Figure | Measurement | Genotype | Total 'n' | Below the limit of detection | Above limit of detection |
||
|---|---|---|---|---|---|---|---|
| n | Mean (pg/mg) | SD | |||||
| 5 A | Guanidine HCl Aβ42/ total brain weight | WT | 4 | 4 | 0 | NA | NA |
| 5 A | Guanidine HCl Aβ42/ total brain weight | Dp3Tyb | NA | NA | NA | NA | NA |
| 5 B | Guanidine HCl Aβ42/total brain weight | WT | 6 | 0 | 6 | 0.505 | 0.444 |
| 5 B | Guanidine HCl Aβ42/ total brain weight | Dp(10)2Tyb | 3 | 0 | 3 | 0.59 | 0.411 |
| 5 C | Guanidine HCl Aβ42/ total brain weight | WT | 5 | 4 | 1 | 6.154 | NA |
| 5 C | Guanidine HCl Aβ42/ total brain weight | Dp(17)3Tyb | 5 | 5 | 0 | NA | NA |
| 5 D | 1% Triton Aβ42/total brain weight | WT | 4 | 3 | 1 | 0.002 | NA |
| 5 D | 1% Triton Aβ42/ total brain weight | Dp3Tyb | NA | NA | NA | NA | NA |
| 5 E | 1% Triton Aβ42/ total brain weight | WT | 6 | 5 | 1 | 0.009 | NA |
| 5 E | 1% Triton Aβ42/ total brain weight | Dp(10)2Tyb | 3 | 2 | 1 | 0.007 | NA |
| 5 F | 1% Triton Aβ42/ total brain weight | WT | 5 | 2 | 3 | 0.475 | 0.814 |
| 5 F | 1% Triton Aβ42/ total brain weight | Dp(17)3Tyb | 5 | 3 | 2 | 0.074 | NA |
| 5 G | Tris Aβ42/ total brain weight | WT | 4 | 3 | 1 | 0.015 | NA |
| 5 G | Tris Aβ42/ total brain weight | Dp3Tyb | NA | NA | NA | NA | NA |
| 5 H | Tris Aβ42/total brain weight | WT | 6 | 5 | 1 | 0.006 | NA |
| 5 H | Tris Aβ42/ total brain weight | Dp(10)2Tyb | 3 | 2 | 1 | 0.007 | NA |
| 5 I | Tris Aβ42/ total brain weight | WT | 5 | 2 | 3 | 0.304 | 0.517 |
| 5 I | Tris Aβ42/ total brain weight | Dp(17)3Tyb | 5 | 2 | 3 | 0.011 | 0.009 |
| 6 A | Guanidine HCl Aβ40/total brain weight | WT | 6 | 0 | 6 | 2.097 | 1.246 |
| 6 A | Guanidine HCl Aβ40/ total brain weight | Dp(10)2Tyb | 3 | 0 | 3 | 2.255 | 1.796 |
| 6 B | Guanidine HCl Aβ40/ total brain weight | WT | 5 | 5 | 0 | NA | NA |
| 6 B | Guanidine HCl Aβ40/ total brain weight | Dp(17)3Tyb | 5 | 5 | 0 | NA | NA |
| 6 C | 1% Triton Aβ42/ total brain weight | WT | 6 | 4 | 2 | 0.058 | 0.004 |
| 6 C | 1% Triton Aβ42/ total brain weight | Dp(10)2Tyb | 3 | 1 | 2 | 0.035 | 0.023 |
| 6 D | 1% Triton Aβ42/ total brain weight | WT | 5 | 4 | 1 | 3.241 | NA |
| 6 D | 1% Triton Aβ42/ total brain weight | Dp(17)3Tyb | 5 | 5 | 5 | NA | NA |
aNegative control samples from mice that do not carry the AppNL-F allele and do not produce human Aβ were assayed by 6E10 MSD assay alongside samples homozygous for the AppNL-F allele and which do produce human Aβ. The epitope for 6E10 lies within the region of sequence difference between human and mouse Aβ; the antibody is against the human sequence. In some cases, as indicated, all samples without an AppNL-F allele were below the limit of detection for the assay; thus, mean and SDs are not available (NA).