Figure 5.
Immunostimulatory RNAs elicit responses with a stronger antiviral component and a lower proinflammatory component
(A and B) Volcano plots showing significant upregulated genes (red) or downregulated genes (blue) in RNA-1 transfected (A) or poly(I:C) transfected (B) A549 cells. Threshold for fold change = 2, threshold for Padj = 0.01. (C) Comparison of the immunostimulatory activities of different RNAs. A549-Dual cells were transfected with indicated duplex RNAs at a 5-fold serial dilution from 50 nM to 16 pM for 24 h, and then activation of the IFN pathway and NF-kB pathway was measured by quantifying luciferase reporter activity or alkaline phosphatase activity, respectively. N = 4. Data are shown as means ± SD.