TABLE 3.
Partitioning of ςA and ςE at T3
| Strain | Protein | % in fractionsa:
|
||
|---|---|---|---|---|
| 1–3 | 4–10 | 11–16 | ||
| Wild type | β | 0 | 87.8 | 12.2 |
| ςA | 0 | 2.0 | 98 | |
| ςE | 0 | 69.4 | 30.6 | |
| Pro-ςE | 0 | 100 | 0 | |
| Spo0A− | β | 0 | 82.7 | 17.3 |
| ςA | 0 | 52.5 | 47.5 | |
| SigE− | β | 1.3 | 84.5 | 14.2 |
| ςA | 0 | 30.5 | 69.5 | |
| SigF− | β | 14.3 | 65.2 | 20.4 |
| ςA | 0 | 32.8 | 67.1 | |
| Pro-ςE | 50.8 | 42.9 | 6.1 | |
| SigE− SigF− | β | 0 | 82.7 | 17.3 |
| ςA | 0 | 29.1 | 70.9 | |
| SigECR117 | β | 6.0 | 79.3 | 14.7 |
| ςA | 0 | 7.5 | 92.5 | |
| ςE | 5.6 | 81.2 | 0 | |
| Pro-ςE | 18.8 | 75.1 | 19.3 | |
a
Percentage of the bound antibody that was detected in a Western blot of the indicated fractions obtained after a nonequilibrium centrifugation experiment (see Materials and Methods). Fraction 1 represents the bottom of the gradient; fraction 16 represents the top.