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Identification of the yrbA promoter. RNAs (20 μg) extracted from wild-type (168) (lane 5) or yrbA (lane 6) cells at T5 of sporulation were used for primer extension. The sizes of the extended products were compared with a DNA sequencing ladder of the adjacent sequence of the 5′ region of yrbA. The yrbA transcription start site is shown by an arrowhead and an asterisk on the sequence.
