FIG. 4.

Genomic structure of the region upstream of yrbA. (A) Nucleotide sequence of the yrbA promoter, showing putative −35 and −10 regions and the transcription start site (+1). The nucleotide sequence complementary to the synthetic oligonucleotide used in primer extension is underlined. The asterisk indicates a translation stop codon. (B) Sequences near the transcription start sites of genes transcribed by RNA polymerase containing ςE. The underlined nucleotides denote the transcription start site. The consensus sequence proposed by Roels et al. (29) is shown at the top (K = G or T; m = C or A; r = G or A). References for the sequences of these promoters are as follows: spoIID, 30; spoIIID, 18 and 43; spoIVF 7; spoIIIA, 8; spoIVA P1 and spoIVA P2, 29; and cotE P1, 48.