FIG. 7.
SDS-PAGE analysis of proteins solubilized from spores. Spores were prepared from T18 sporulating cells. The protein samples were solubilized from the spores by boiling with SDS and 2-mercaptoethanol and analyzed by SDS-PAGE (12% gel). (A) Coomassie brilliant blue stain; (B) immunoblotting with anti-YrbB antibody. The protein samples were from wild-type spores (lanes 1), TB711 (yrbB) spores (lanes 2), and TB713 (yrbA) spores (lanes 3). The arrowhead in panel B indicates the migration position of YrbB.