TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Source (reference) |
|---|---|---|
| B. subtilis | ||
| 168 | trpC2 | Laboratory stock |
| SC1159 | spoIIAC1 (SigF mutant) | S. Cutting (7) |
| 1S60 | leuB8 tal-1 spoIIG41 (SigE mutant) | BGSCa |
| spoIIIGΔ1 | trpC2 spoIIIGΔ1 (SigG mutant) | J. Sekiguchi (36) |
| 1S38 | trpC2 spoIIIC94 (SigK mutant) | BGSC |
| TB711 | trpC2 yrbB::cat | This work |
| TB713 | trpC2 yrbA::cat | This work |
| E. coli JM109 | relA supE44 endA1 hsdR17 gyrA96 mcrA mcrB+ thiΔ(lac-proAB)/F′ (traD36 proAB+ lacIq lacZΔM15) | Sambrook et al. (33) |
| Plasmids | ||
| pDH88 | Cmr AmprPspac-1 | D. Henner (12) |
| pCOXA5 | Cmr AmprPspac-1 yrbB′ | This work |
| pYRBA5 | Cmr AmprPspac-1 yrbA′ | This work |
a
BGSC, Bacillus Genetic Stock Center.