Figure 2.

Characterization of prognostic implications and biological functions of 23 m⁶A regulators across HCC: (a) functional annotations of the regulators with GO enrichment analyses. The bar length represented the number of enriched regulators. (b) The interaction of expression on 23 m⁶A regulators (writers, yellow; readers, blue; erasers, red) in HCC. The lines that connected modifiers indicated their interactions, and the circle size indicated the survival value of regulators and scaled by p values. (c) Uni- and (d) multivariate cox regression analyses of 23 m⁶A regulators in HCC prognosis. (e, f) Western blot of the expression of ZC3H13 in HeG2 and HUH-7 cells transfected with empty vector and ZC3H13 up-regulation. (g, h) EdU staining for cell proliferation of HeG2 and HUH-7 cells with empty vector and ZC3H13 up-regulation. (i–l) Transwell assays for the migration and invasion of HeG2 and HUH-7 cell lines following transfection with empty vector and ZC3H13 overexpression. (m) Heatmap showing the top 30 genes associated with ZC3H13 in HCC. ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001.